![]() Additional data are archived through the Whitman College Penrose Library Digital Archive ( ).įunding: Support was provided by the Eunice Kennedy Shriver National Institute of Child Health and Human Development (1R15HD061831) and the National Science Foundation (DBI-1039958 and DBI-0922978). This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the paper and its supporting information files. Received: JAccepted: DecemPublished: January 12, 2017Ĭopyright: © 2017 Withers et al. ![]() PLoS ONE 12(1):Įditor: Thierry Amédée, Centre National de la Recherche Scientifique, FRANCE Taken together, these observations reveal influences on spatial orientation of growth as well as influences on morphogenesis of the dendritic arbor that have not been previously identified.Ĭitation: Withers GS, Farley JR, Sterritt JR, Crane AB, Wallace CS (2017) Interactions with Astroglia Influence the Shape of the Developing Dendritic Arbor and Restrict Dendrite Growth Independent of Promoting Synaptic Contacts. Thus, while astroglia promoted the formation of presynaptic contacts onto dendrites, dendritic growth was constrained locally within a developing arbor at sites where dendrites contacted astroglia. Notably, thrombospondin, the astroglial factor shown previously to promote synapse formation, did not inhibit dendritic growth. Further, dendritic arbors in partial physical contact with astroglia developed a pronounced pattern of asymmetrical growth, because the dendrites in direct contact were significantly smaller than the portion of the arbor not in contact. ![]() When astroglia were proximal to neurons, dendrites and dendritic filopodia oriented toward them, but the general presence of astroglia significantly reduced overall dendrite growth. Here, we used cultured hippocampal neurons as a simple in vitro model that allowed dendritic growth and geometry to be analyzed quantitatively under conditions where the extent of interactions between neurons and astrocytes varied. Astroglia play key roles in the development of neurons, ranging from regulating neuron survival to promoting synapse formation, yet basic questions remain about whether astrocytes might be involved in forming the dendritic arbor. ![]()
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